A Method for Normalization of Gene Expression Data

Advances in microarray technologies have made it possible to comprehensively measure gene expression profiles. However, in these experiments, the efficiency of fluorescent dyes are different between different experiments, and adjustments of these expression values are mandatory before the analysis, It is referred to as normalization. Although the most popular method is total intensity normalization, where each fluorescent intensity value is divided by the sum of all the fluorescent intensities, it is difficult to apply this method in case that the expression of some of the genes changes too much. In this paper, we describe a novel method for normalizing gene expression data of microarays so that we can compare results of microarray experiments correctly even in these difficult cases.